3BRT
NEMO/IKK association domain structure
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794,0.9799 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.869, 105.556, 56.707 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.900 - 2.250 |
| R-factor | 0.23961 |
| Rwork | 0.238 |
| R-free | 0.28186 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.928 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.072 | 0.396 |
| Number of reflections | 11760 | |
| <I/σ(I)> | 1.7 | |
| Completeness [%] | 93.4 | 69.4 |
| Redundancy | 7.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 8 | 298 | 35% Peg 8K, 0.1M Tris pH 8, 0.2M Ammonium Chloride, 10mM Dithiothreitol., microbatch under oil, temperature 298K |
