3BRG
CSL (RBP-Jk) bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-17 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 66.769, 95.388, 113.695 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.060 - 2.200 |
| R-factor | 0.22079 |
| Rwork | 0.219 |
| R-free | 0.25515 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.635 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0020) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.049 | 0.034 | 0.271 |
| Number of reflections | 37593 | ||
| <I/σ(I)> | 19.2 | ||
| Completeness [%] | 99.9 | 99.1 | 100 |
| Redundancy | 6.7 | 6.4 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | under oil microbatch | 5.5 | 277 | Bis-Tris, Sodium Chloride, PEG 3350, Xylitol, pH 5.5, under oil microbatch, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Bis | ||
| 10 | 1 | 2 | Xylitol | ||
| 2 | 1 | 1 | Tris | ||
| 3 | 1 | 1 | Sodium Chloride | ||
| 4 | 1 | 1 | PEG 3350 | ||
| 5 | 1 | 1 | Xylitol | ||
| 6 | 1 | 2 | Bis | ||
| 7 | 1 | 2 | Tris | ||
| 8 | 1 | 2 | Sodium Chloride | ||
| 9 | 1 | 2 | PEG 3350 |
