3BRD
CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, P212121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-30 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.153, 98.866, 126.313 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.870 - 2.210 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.795 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0020) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.066 | 0.042 | 0.345 |
| Number of reflections | 36557 | ||
| <I/σ(I)> | 14.7 | ||
| Completeness [%] | 94.8 | 99 | 71.3 |
| Redundancy | 5.5 | 5.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | under oil microbatch | 5.5 | 277 | Bis-Tris, Ammonium Sulfate, PEG 3350, Ethylene Glycol, pH 5.5, under oil microbatch, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Bis | ||
| 10 | 1 | 2 | Ethylene Glycol | ||
| 2 | 1 | 1 | Tris | ||
| 3 | 1 | 1 | Ammonium Sulfate | ||
| 4 | 1 | 1 | PEG 3350 | ||
| 5 | 1 | 1 | Ethylene Glycol | ||
| 6 | 1 | 2 | Bis | ||
| 7 | 1 | 2 | Tris | ||
| 8 | 1 | 2 | Ammonium Sulfate | ||
| 9 | 1 | 2 | PEG 3350 |
