3BQU

Crystal Structure of the 2F5 Fab'-3H6 Fab Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2004-11-25
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	67.440, 98.330, 154.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.276
Rwork	0.276
R-free	0.29500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2F5B 1f11 1acy
RMSD bond length	0.004
RMSD bond angle	1.002
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.050
High resolution limit [Å]	3.000	3.000
Number of reflections	19786
<I/σ(I)>	15.2	4
Completeness [%]	93.6	94.2
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	15-18% PEG 8000, 0.20 M ammonium sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K