3BQ7
SAM domain of Diacylglycerol Kinase delta1 (E35G)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.27 |
| Spacegroup name | P 32 |
| Unit cell lengths | 108.079, 108.079, 33.513 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.040 - 2.900 |
| R-factor | 0.254 |
| Rwork | 0.250 |
| R-free | 0.29000 |
| Starting model (for MR) | 2f3n |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.250 | 2.900 |
| Rmerge | 0.064 | 0.051 | 0.344 |
| Number of reflections | 18986 | ||
| <I/σ(I)> | 17.6 | ||
| Completeness [%] | 98.1 | 98.8 | 95.4 |
| Redundancy | 2 | 2.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | dibasic ammonium phosphate, tris, NaCl, beta-mercaptoethanol, pH 8.0, vapor diffusion, hanging drop, temperature 298K |
