3BQ4

Crystal Structure of Ad35 fiber knob

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Wavelength(s)	0.979
Spacegroup name	I 4
Unit cell lengths	173.906, 173.906, 154.145
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.030 - 2.700
R-factor	0.332
Rwork	0.332
R-free	0.35200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.700
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.700
Rmerge	0.149
Number of reflections	85255
<I/σ(I)>	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295.5	1.6 M Ammonium sulfate and 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K