3BP1
Crystal structure of putative 7-cyano-7-deazaguanine reductase QueF from Vibrio cholerae O1 biovar eltor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-29 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.517, 71.578, 71.511 |
| Unit cell angles | 119.25, 110.18, 99.58 |
Refinement procedure
| Resolution | 26.160 - 1.530 |
| R-factor | 0.145 |
| Rwork | 0.143 |
| R-free | 0.18300 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.578 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Number of reflections | 149957 | |
| <I/σ(I)> | 7.5 | 2.4 |
| Completeness [%] | 94.1 | 76.5 |
| Redundancy | 4.5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.04M Sodium dihydrogen phosphate, 0.96M Di-potassium hydrogen phosphate, GTP, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
