3BO5
Crystal structure of methyltransferase domain of human Histone-lysine N-methyltransferase SETMAR
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793143, 1.2832396 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.396, 67.827, 44.637 |
| Unit cell angles | 90.00, 105.93, 90.00 |
Refinement procedure
| Resolution | 42.910 - 1.590 |
| R-factor | 0.15741 |
| Rwork | 0.155 |
| R-free | 0.19889 |
| Structure solution method | MAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.057 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.910 | 1.660 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Number of reflections | 31726 | |
| <I/σ(I)> | 27 | 2.5 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 4.9 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 25% PEG 3350, 0.2 M Ammonium acetate, 0.1 M Bis-Tris pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
