3BNR
Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site in the presence of nonspecifically bound paromomycin (A1555G mutant, Br-derivative)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.91625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.189, 77.380, 56.322 |
| Unit cell angles | 90.00, 115.12, 90.00 |
Refinement procedure
| Resolution | 50.995 - 2.100 |
| Rwork | 0.215 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.900 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.995 | 50.990 | 2.210 |
| High resolution limit [Å] | 2.100 | 6.640 | 2.100 |
| Rmerge | 0.116 | 0.089 | 0.378 |
| Total number of observations | 3289 | 16679 | |
| Number of reflections | 16388 | ||
| <I/σ(I)> | 2.7 | 6 | 1.5 |
| Completeness [%] | 99.8 | 96 | 100 |
| Redundancy | 6.9 | 6.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium cacodylate, KCl, spermine tetrachloride, paromomycin, 2-methyl-2,4-pentanediol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | Sodium cacodylate | ||
| 4 | 1 | 2 | MPD | ||
| 5 | 1 | 2 | KCl |
