3BNO
Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site (Br-derivative)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-08 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8856 |
Spacegroup name | P 1 |
Unit cell lengths | 35.140, 45.460, 52.130 |
Unit cell angles | 71.43, 73.42, 74.05 |
Refinement procedure
Resolution | 36.880 - 2.350 |
Rwork | 0.226 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.901 |
Data scaling software | d*TREK (9.2SSI) |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 36.880 | 36.880 | 2.430 |
High resolution limit [Å] | 2.350 | 5.060 | 2.350 |
Rmerge | 0.038 | 0.029 | 0.366 |
Total number of observations | 8691 | 9087 | |
Number of reflections | 11751 | ||
<I/σ(I)> | 17.8 | 45.9 | 3.2 |
Completeness [%] | 97.8 | 92.9 | 97.7 |
Redundancy | 3.93 | 3.82 | 3.97 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium cacodylate, spermine tetrachloride, paromomycin, KCl, 2-methyl-2,4-pentanediol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | Sodium cacodylate | ||
4 | 1 | 2 | MPD | ||
5 | 1 | 2 | KCl |