3BNN
Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.610, 44.500, 52.360 |
| Unit cell angles | 68.77, 71.30, 67.72 |
Refinement procedure
| Resolution | 39.590 - 2.000 |
| Rwork | 0.238 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.910 |
| Data scaling software | d*TREK (9.2SSI) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.590 | 39.590 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.031 | 0.019 | 0.369 |
| Total number of observations | 4634 | 4292 | |
| Number of reflections | 17270 | ||
| <I/σ(I)> | 14.6 | 46.7 | 2.5 |
| Completeness [%] | 97.0 | 96.2 | 92.6 |
| Redundancy | 2.67 | 2.68 | 2.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium cacodylate, KCl, spermine tetrachloride, paromomycin, 2-methyl-2,4-pentanediol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | Sodium cacodylate | ||
| 4 | 1 | 2 | MPD | ||
| 5 | 1 | 2 | KCl |
