3BMX
Beta-N-hexosaminidase (YbbD) from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-04-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99989 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.385, 73.085, 83.567 |
| Unit cell angles | 79.79, 69.61, 88.25 |
Refinement procedure
| Resolution | 42.835 - 1.400 |
| R-factor | 0.129 |
| Rwork | 0.127 |
| R-free | 0.16600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries: 1EX1 1IIX 1j8v 1lq2 1x38 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.967 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.084 | 0.605 |
| Number of reflections | 227962 | |
| <I/σ(I)> | 13.25 | 2.65 |
| Completeness [%] | 90.8 | 53.7 |
| Redundancy | 7 | 2.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 291 | 0.1M sodium acetate, 30-33 % PEG 1000, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
