3BM6
AmpC beta-lactamase in complex with a p.carboxyphenylboronic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Detector technology | CCD |
| Collection date | 2005-01-12 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.908, 77.180, 97.937 |
| Unit cell angles | 90.00, 115.98, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.25800 |
| Starting model (for MR) | 1ga9 |
| RMSD bond length | 0.006 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.055 |
| Number of reflections | 46573 |
| <I/σ(I)> | 15.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 295 | potassium phosphate buffer, pH 8.7, vapor diffusion, hanging drop, temperature 295K |
