3BLX
Yeast Isocitrate Dehydrogenase (Apo Form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 112.444, 115.208, 159.247 |
| Unit cell angles | 111.03, 96.08, 107.13 |
Refinement procedure
| Resolution | 35.739 - 2.700 |
| R-factor | 0.2407 |
| Rwork | 0.239 |
| R-free | 0.26430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3blv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.275 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 185029 | |
| <I/σ(I)> | 13.7 | 2 |
| Completeness [%] | 96.6 | 94.1 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 16% polyethylene glycol 3350, 0.13 M potassium sulfate, 0.2 M trimethylamine-N-oxide, 15% propanediol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
