3BLV
Yeast Isocitrate Dehydrogenase with Citrate Bound in the Regulatory Subunits
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791, 0.9796 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 256.899, 112.949, 125.616 |
| Unit cell angles | 90.00, 106.67, 90.00 |
Refinement procedure
| Resolution | 49.346 - 3.200 |
| R-factor | 0.2235 |
| Rwork | 0.221 |
| R-free | 0.26490 |
| Structure solution method | MAD and MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wpw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.149 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 109531 | |
| <I/σ(I)> | 11 | 2.1 |
| Completeness [%] | 96.8 | 97.9 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 295 | 1.1 M sodium citrate, 0.1 M CHES, 15% ethylene glycol, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
