3BKM
Structure of anti-amyloid-beta Fab WO2 (Form A, P212121)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-06 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.447, 90.496, 123.279 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.600 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.22600 |
| Starting model (for MR) | 1plg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.476 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.940 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.598 |
| Rmerge | 0.101 | 0.101 |
| Number of reflections | 76623 | |
| <I/σ(I)> | 16.6 | 16.6 |
| Completeness [%] | 98.0 | 88.08 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 100mM MES, 10mM ZnSO4, 20% (v/v)PEG 550 monoethylether, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
