3BKB
Crystal structure of human Feline Sarcoma Viral Oncogene Homologue (v-FES)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98074 |
| Spacegroup name | P 63 |
| Unit cell lengths | 80.404, 80.404, 131.411 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.810 - 1.780 |
| R-factor | 0.15 |
| Rwork | 0.148 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | An ensemble of three kinase domains from the PDB entries 2HYY 2g2i 2g2h |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.532 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.810 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.128 | 0.787 |
| Number of reflections | 46019 | |
| <I/σ(I)> | 18.7 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2M Na2SO4, 0.1M Bis-Tris-Propane, 20% PEG 3350, 10% Ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
