3BK8
Urate oxidase aza-xanthine complex in cyanide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 290 |
Detector technology | CCD |
Collection date | 2007-05-12 |
Detector | MARRESEARCH |
Wavelength(s) | 0.978 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 79.718, 95.105, 103.957 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.600 |
R-factor | 0.2002 |
Rwork | 0.200 |
R-free | 0.23010 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2icq |
RMSD bond length | 0.015 |
RMSD bond angle | 0.028 |
Data reduction software | MOSFLM |
Phasing software | FFT |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.055 | 0.210 |
Number of reflections | 50567 | |
<I/σ(I)> | 22.3 | 4.4 |
Completeness [%] | 98.9 | 99.9 |
Redundancy | 4.3 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 289 | 8.5MG/ML URATE OXIDASE, 0.2 M SODIUM CYANIDE, 50MM TRIS BUFFER, 0.5MG/ML 8-AZAXANTHINE, 15% PEG 8000 , pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |