3BK6
Crystal structure of a core domain of stomatin from Pyrococcus horikoshii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-24 |
| Detector | ADSC QUAMTUM 4r |
| Wavelength(s) | 0.9791, 0.9796, 0.9900, 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.553, 137.150, 58.544 |
| Unit cell angles | 90.00, 107.61, 90.00 |
Refinement procedure
| Resolution | 19.970 - 3.200 |
| R-factor | 0.20446 |
| Rwork | 0.198 |
| R-free | 0.26234 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.176 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.058 | 0.474 |
| Number of reflections | 17735 | |
| <I/σ(I)> | 33.5 | 3.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% MPD, 0.2M ammonium sulfate, 0.1M HEPES-NaOH, 6% 1,5-diaminopentane-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
