3BJF

Pyruvate kinase M2 is a phosphotyrosine binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 1
Unit cell lengths	80.756, 81.458, 106.885
Unit cell angles	74.63, 69.91, 66.10

Refinement procedure

Resolution	44.290 - 2.030
R-factor	0.208
Rwork	0.208
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	143996
<I/σ(I)>	28.9
Completeness [%]	96.3
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1