3BJ7

Spermine/spermidine N1-acetyltransferase from mouse: Crystal structure of a ternary complex reveals solvent-mediated spermine binding

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-15
Detector	MAR CCD 165 mm
Wavelength(s)	1.38
Spacegroup name	P 21 21 21
Unit cell lengths	76.857, 97.191, 105.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.400 - 2.200
R-factor	0.231
Rwork	0.231
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.400
High resolution limit [Å]	2.100
Number of reflections	46525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K