3BIS
Crystal Structure of the PD-L1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-08 |
| Detector | SBC-3 |
| Wavelength(s) | 1.0722, 0.97948, 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.080, 92.700, 141.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.840 - 2.640 |
| R-factor | 0.22 |
| Rwork | 0.217 |
| R-free | 0.29200 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.526 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.730 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.059 | 0.272 |
| Number of reflections | 14169 | |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 95.9 | |
| Redundancy | 5.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 20% PEG 3350, 0.1 M NaCacodylate, 0.2 M ammonium formate or ammonium fluoride, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
