3BIL
Crystal structure of a probable LacI family transcriptional regulator from Corynebacterium glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-17 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.209, 54.338, 78.650 |
| Unit cell angles | 90.00, 94.19, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.221 |
| Rwork | 0.218 |
| R-free | 0.27800 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.698 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.922 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.130 | 0.616 |
| Number of reflections | 22200 | |
| <I/σ(I)> | 16.6 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 30% PEG 3350, 150mM Ammonium fluoride, pH 7.0, VAPOR DIFFUSION, temperature 294K |
