3BIF
6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE EMPTY 6-PF-2K ACTIVE SITE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1997-10-09 |
Detector | MACSCIENCE |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 106.700, 108.700, 71.700 |
Unit cell angles | 90.00, 129.50, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.197 * |
Rwork | 0.197 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bif |
RMSD bond length | 0.008 |
RMSD bond angle | 21.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.039 * | |
Number of reflections | 23883 | |
<I/σ(I)> | 36.5 | 7.16 |
Completeness [%] | 88.1 | 84.9 |
Redundancy | 2.64 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 4 * | PROTEIN WAS CRYSTALLIZED FROM 50MM SUCCINATE, PH 5.5, 50MM TRIS-PO4, PH 7.5, 11% PEG 4000, 100UM EDTA, 10MM DTT, 10% GLYCEROL, 3MM MGCL2., pH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | RT2K-Wo | 10 (mg/ml) | |
10 | 1 | reservoir | succinate | 50 (mM) | |
11 | 1 | reservoir | Tris-PO4 | 50 (mM) | |
12 | 1 | reservoir | EDTA | 0.100 (mM) | |
13 | 1 | reservoir | dithiothreitol | 10 (mM) | |
14 | 1 | reservoir | glycerol | 10 (%) | |
15 | 1 | reservoir | 3 (mM) | ||
2 | 1 | drop | Tris-PO4 | 50 (mM) | |
3 | 1 | drop | glycerol | 5 (%) | |
4 | 1 | drop | EDTA | 0.100 (mM) | |
5 | 1 | drop | dithiothreitol | 10 (mM) | |
6 | 1 | drop | octyl beta-D-glucopyranoside | 0.5 (%) | |
7 | 1 | drop | F6P | 3 (mM) | |
8 | 1 | drop | AMP-PNP | 1 (mM) | |
9 | 1 | reservoir | PEG4000 | 11 (%) |