3BHS
Nitrosomonas europaea Rh50 and mechanism of conduction by Rhesus protein family of channels
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 78 |
Detector technology | CCD |
Collection date | 2007-03-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.11587 |
Spacegroup name | H 3 |
Unit cell lengths | 96.675, 96.675, 137.621 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.060 - 1.990 |
R-factor | 0.1963 |
Rwork | 0.196 |
R-free | 0.23666 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ns1 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.487 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 1.990 | 1.990 |
Number of reflections | 32561 | |
<I/σ(I)> | 14.7 | 2.4 |
Completeness [%] | 99.8 | 98.2 |
Redundancy | 6.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1:1 microliter drop ratio of (Protein in 0.02M HEPES pH 7.4, 0.1M NaCl, 0.04M B-octyl glucoside, 2mM Dithiothreitol) and (100mM HEPES, 25% PEG 400). Cryoprotected with 10% Glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |