3BHG
Crystal structure of adenylosuccinate lyase from Legionella pneumophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97857 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 109.216, 109.216, 157.099 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.710 - 1.900 |
R-factor | 0.16772 |
Rwork | 0.166 |
R-free | 0.19529 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.309 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.078 | 0.571 |
Number of reflections | 37453 | |
<I/σ(I)> | 59 | 4.75 |
Completeness [%] | 99.5 | 98.5 |
Redundancy | 18.3 | 16.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1 M Bis-tris, 2.0 M Ammonium sulfate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |