3BGT
Structural Studies of Acetoacetate Decarboxylase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-28 |
| Detector | Quantum-315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | H 3 |
| Unit cell lengths | 105.447, 105.447, 252.379 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.300 - 2.100 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.24500 |
| Structure solution method | SAD |
| RMSD bond length | 0.029 |
| RMSD bond angle | 1.961 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.300 |
| High resolution limit [Å] | 1.990 |
| Number of reflections | 133725 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.7 | 291 | 0.5M potassium phosphate dibasic/sodium phoshpate monobasic, pH 7.7, hanging drop, temperature 291K |
