3BG7
Pyranose 2-oxidase from Trametes multicolor, L537G mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.042 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 168.540, 103.180, 169.340 |
| Unit cell angles | 90.00, 106.45, 90.00 |
Refinement procedure
| Resolution | 29.720 - 2.100 |
| R-factor | 0.15645 |
| Rwork | 0.156 |
| R-free | 0.20395 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2igo |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.863 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 321136 | |
| <I/σ(I)> | 17.2 | 6.2 |
| Completeness [%] | 99.0 | 94 |
| Redundancy | 4.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 12-14(w/v)% monomethylether PEG 2000, 0.1M Mes, 50mM MgCl2, 25% glycerol, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
