3BG6
Pyranose 2-oxidase from Trametes multicolor, E542K mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 168.863, 103.746, 169.333 |
| Unit cell angles | 90.00, 106.31, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.700 |
| R-factor | 0.16601 |
| Rwork | 0.166 |
| R-free | 0.19787 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2igo |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.887 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 603616 | |
| <I/σ(I)> | 9.7 | 2.2 |
| Completeness [%] | 98.2 | 97.4 |
| Redundancy | 3.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 12-14(w/v)% monomethylether PEG 2000, 0.1M Mes, 50mM MgCl2, 25% glycerol, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
