3BG3
Crystal Structure of Human Pyruvate Carboxylase (missing the biotin carboxylase domain at the N-terminus)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.274, 173.321, 118.194 |
| Unit cell angles | 90.00, 95.87, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.21852 |
| Rwork | 0.216 |
| R-free | 0.27129 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.228 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.072 | 0.413 |
| Number of reflections | 76525 | |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 94.3 | 67.4 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 90 mM MnCl2, 0.8%(v/v)PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
