3BFO
Crystal structure of Ig-like C2-type 2 domain of the human Mucosa-associated lymphoid tissue lymphoma translocation protein 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-05 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91790 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.944, 43.640, 49.595 |
| Unit cell angles | 66.50, 90.00, 90.02 |
Refinement procedure
| Resolution | 25.000 - 1.150 |
| R-factor | 0.17882 |
| Rwork | 0.177 |
| R-free | 0.21182 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1U2H 2aw2 1cs6 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.830 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.190 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Number of reflections | 103829 | |
| <I/σ(I)> | 22 | 2.48 |
| Completeness [%] | 94.6 | 91.8 |
| Redundancy | 5.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1:1 volume ratio of protein solution (50 mg/mL) and reservoir solution (30% PEG MME 550, 0.2 M Ammonium sulfate, 0.1 M Sodium cacodylate pH 6.5), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
