3BF5

Crystal structure of putative ribokinase (10640157) from Thermoplasma acidophilum at 1.91 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.91840, 0.97939, 0.97953
Spacegroup name	P 21 21 21
Unit cell lengths	56.450, 59.180, 177.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.185 - 1.910
R-factor	0.205
Rwork	0.202
R-free	0.24500
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.596
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.185	29.185	1.980
High resolution limit [Å]	1.910	4.100	1.910
Rmerge	0.050	0.017	0.685
Number of reflections	46740	8401	9105
<I/σ(I)>	10.78	37.8	1.4
Completeness [%]	98.4	92.8	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	NANODROP, 40.0% 1,2-propanediol, 0.1M Acetate pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K