3BF2
Crystal structure of the A1KSW9_NEIMF protein from Neisseria meningitidis. Northeast Structural Genomics Consortium target MR36a
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97910, 0.97940, 0.96785 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 65.296, 65.296, 92.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.310 - 2.600 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.25800 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXDE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.067 | 0.463 |
Number of reflections | 11698 | |
<I/σ(I)> | 36.89 | 6.72 |
Completeness [%] | 99.0 | 99.8 |
Redundancy | 15.1 | 15.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 2.5 M Li chloride, 0.1 M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |