3BEZ
Crystal structure of Escherichia coli Signal peptide peptidase (SppA), SeMet crystals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.022, 153.040, 100.214 |
| Unit cell angles | 90.00, 104.17, 90.00 |
Refinement procedure
| Resolution | 46.630 - 2.760 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.25900 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.892 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.900 | 2.900 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmerge | 0.178 | 0.450 |
| Number of reflections | 69169 | |
| <I/σ(I)> | 15.6 | 3.3 |
| Completeness [%] | 97.8 | 78 |
| Redundancy | 7.4 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG3350, pH 7.5, vapor diffusion, hanging drop, temperature 291K |
