3BER
Human DEAD-box RNA-helicase DDX47, conserved domain I in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97472 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.050, 70.370, 35.860 |
| Unit cell angles | 90.00, 90.70, 90.00 |
Refinement procedure
| Resolution | 28.240 - 1.400 |
| R-factor | 0.16659 |
| Rwork | 0.165 |
| R-free | 0.18914 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gxs |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.638 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.052 | 0.141 |
| Number of reflections | 45260 | |
| <I/σ(I)> | 28.7 | 14.1 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 277 | 42.5% PEG 200, 100mM Sodium/potassium phosphate, 50mM Sodium chloride, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
