3BEH

Structure of a Bacterial Cyclic Nucleotide Regulated Ion Channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Detector technology	CCD
Collection date	2006-09-15
Wavelength(s)	1.08
Spacegroup name	H 3
Unit cell lengths	282.730, 282.730, 105.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.450 - 3.100
R-factor	0.276
Rwork	0.276
R-free	0.28600
RMSD bond length	0.006
RMSD bond angle	0.898
Data scaling software	XSCALE
Refinement software	REFMAC (refmac_5.2.0019 24/04/2001)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.450	3.180
High resolution limit [Å]	3.100	3.100
Number of reflections	56604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	PEG2000, pH 6.0, temperature 298K, VAPOR DIFFUSION, SITTING DROP