3BEG
Crystal structure of SR protein kinase 1 complexed to its substrate ASF/SF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 57.406, 117.525, 193.554 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.400 - 2.900 |
| R-factor | 0.24 |
| Rwork | 0.237 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.493 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.976 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 13862 | |
| <I/σ(I)> | 19.9 | 2.54 |
| Completeness [%] | 93.0 | 62.2 |
| Redundancy | 4.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 0.1M sodium citrate, 0.2M sodium acetate, 5% ethanol, 15% PEGMME 5000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
