3BDU
Crystal structure of protein Q6D8G1 at the resolution 1.9 A. Northeast Structural Genomics Consortium target EwR22A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-03 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.974, 79.077, 48.472 |
| Unit cell angles | 90.00, 96.30, 90.00 |
Refinement procedure
| Resolution | 19.800 - 1.900 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.24700 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.230 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.069 | 0.358 |
| Number of reflections | 61408 | |
| <I/σ(I)> | 17.1 | 3.6 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 4.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.15 | 293 | 25% PEG 400, 0.1M Ca Acetate, 0.1M MES, pH 6.15, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
