3BDL
Crystal structure of a truncated human Tudor-SN
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2007-03-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979389, 0.979545, 0.964305 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.405, 91.929, 88.042 |
| Unit cell angles | 90.00, 91.26, 90.00 |
Refinement procedure
| Resolution | 23.330 - 1.900 |
| Rwork | 0.175 |
| R-free | 0.20900 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.330 | 1.890 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Number of reflections | 68052 | |
| <I/σ(I)> | 15 | 2.4 |
| Completeness [%] | 97.8 | 97.3 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 283 | 50mM HEPES, pH 7.0, 150mM NaCl, 10mM mercaptoethanol, 1.44M tri-ammonium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
