3BDE

Crystal structure of a dabb family protein with a ferredoxin-like fold (mll5499) from mesorhizobium loti maff303099 at 1.79 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9537, 0.9797, 0.9796
Spacegroup name	C 1 2 1
Unit cell lengths	118.669, 28.415, 76.985
Unit cell angles	90.00, 117.78, 90.00

Refinement procedure

Resolution	29.540 - 1.790
R-factor	0.179
Rwork	0.177
R-free	0.22400
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.620
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.3.0040)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.540	29.540	1.840
High resolution limit [Å]	1.790	8.010	1.790
Rmerge	0.074	0.053	0.465
Total number of observations		593	1847
Number of reflections	20638
<I/σ(I)>	7.8	8.8	1.7
Completeness [%]	94.5	93.9	73.2
Redundancy	2.3	2.2	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.9	277	NANODROP, 0.2M Na Acetate, 20.0% PEG 3350, No Buffer pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 277K