3BD6
Glycogen Phosphorylase complex with 1(-D-ribofuranosyl) cyanuric acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2005-10-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.81 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.620, 128.620, 116.379 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.580 - 2.000 |
| R-factor | 0.19092 |
| Rwork | 0.190 |
| R-free | 0.20515 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2prj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.023 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 65282 | |
| <I/σ(I)> | 18.6 | 4.5 |
| Completeness [%] | 98.5 | 98.1 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 10mM Bes buffer, 3mM DDT, pH6.7, SMALL TUBES, temperature 289K |
