3BD1
Structure of the Cro protein from putative prophage element Xfaso 1 in Xylella fastidiosa strain Ann-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9002 |
Spacegroup name | P 32 |
Unit cell lengths | 66.970, 66.970, 54.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 17.060 - 1.400 |
R-factor | 0.147 |
Rwork | 0.147 |
R-free | 0.18200 |
Structure solution method | ACORN (DDM) |
Starting model (for MR) | 2hin |
RMSD bond length | 0.010 |
RMSD bond angle | 1.792 |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.2SSI) |
Phasing software | ACORN |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 58.030 | 17.060 | 1.450 |
High resolution limit [Å] | 1.400 | 3.010 | 1.400 |
Rmerge | 0.048 | 0.025 | 0.386 |
Total number of observations | 15717 | 19627 | |
Number of reflections | 53726 | ||
<I/σ(I)> | 13.3 | 44.3 | 3.2 |
Completeness [%] | 98.6 | 87.2 | 99.7 |
Redundancy | 3.61 | 3.25 | 3.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 16% glycerol, 1.5 M ammonium sulfate, 0.1 M Tris, 1 mM TCEP-hydrochloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |