3BD0
Crystal structure of Memo, form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Detector technology | CCD |
| Collection date | 2007-10-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97989 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 139.877, 88.307, 97.051 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.680 - 3.010 |
| R-factor | 0.217 |
| Rwork | 0.213 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bcz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.330 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.440 | 3.000 |
| Rmerge | 0.227 | 0.084 | 0.626 |
| Number of reflections | 24559 | ||
| <I/σ(I)> | 3.5 | ||
| Completeness [%] | 99.9 | 99.7 | 99.8 |
| Redundancy | 7.2 | 6.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Crystal was grown in 22.5% PEG 3350, 0.1 M MES pH 5.5. Soaked in 5 mM ZnCl2, 22.5% PEG 3350 pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
