3BCE
Crystal structure of the ErbB4 kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Detector technology | IMAGE PLATE |
Collection date | 2007-08-25 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.97893 |
Spacegroup name | P 32 |
Unit cell lengths | 86.723, 86.723, 120.008 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.198 |
Rwork | 0.195 |
R-free | 0.25100 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.297 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
Rmerge | 0.107 | 0.049 | 0.457 |
Number of reflections | 34855 | ||
<I/σ(I)> | 6.1 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 2.6 | 2.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 15% PEG3350, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |