3BBF
Crystal structure of the NM23-H2 transcription factor complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.1271 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.750, 86.780, 159.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.700 |
R-factor | 0.19811 |
Rwork | 0.196 |
R-free | 0.23592 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nue |
RMSD bond length | 0.017 |
RMSD bond angle | 1.669 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.062 | 0.330 |
Number of reflections | 103223 | |
<I/σ(I)> | 25.4 | 3.3 |
Completeness [%] | 96.6 | 81.4 |
Redundancy | 6.5 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 283 | PEG 1500, 50 mM Tris-HCl, 200 mM MgCl2, 2 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |