3BBB
Crystal structure of the NM23-H2 transcription factor complex with dinucleotide d(AG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-13 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.466, 118.219, 128.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.000 - 1.300 |
R-factor | 0.17696 |
Rwork | 0.175 |
R-free | 0.22025 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nue |
RMSD bond length | 0.020 |
RMSD bond angle | 1.885 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.000 | 1.350 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.086 | 0.420 |
Number of reflections | 195789 | |
<I/σ(I)> | 16.4 | 2.3 |
Completeness [%] | 99.3 | 98.3 |
Redundancy | 8 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 1500, 50 mM sodium citrate, 20 mM MgCl2, 5 mM DTT, Crystals soaked with 10 mM d(AG), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |