3BB2
Crystal structure of L26D/D28N mutant of Human acidic fibroblast growth factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2006-10-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 75.442, 96.700, 108.071 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.890 - 1.500 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jqz |
RMSD bond length | 0.017 |
RMSD bond angle | 1.800 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.890 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.060 | 0.399 |
Number of reflections | 56349 | |
<I/σ(I)> | 47.31 | 5.2 |
Completeness [%] | 92.0 | 100 |
Redundancy | 6.8 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | 2.8M Na-formate, 0.6M (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |