3BAE
Crystal structure of Fab WO2 bound to the N terminal domain of Amyloid beta peptide (1-28)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.658, 66.365, 115.337 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.380 - 1.593 |
| R-factor | 0.20897 |
| Rwork | 0.207 |
| R-free | 0.24968 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1plg |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.553 |
| Data reduction software | MOSFLM |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.383 | 1.634 |
| High resolution limit [Å] | 1.590 | 1.593 |
| Rmerge | 0.073 | 0.030 |
| Number of reflections | 53254 | |
| <I/σ(I)> | 11.4 | 3.8 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.49 | 3.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 100mM bicine, 200mM MgCl2, 20% w/v PEG 8000 , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
