3BAD
Crystal structure of D70A/H93G mutant of Human acidic fibroblast growth factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2006-11-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.212, 111.085, 36.835 |
| Unit cell angles | 90.00, 117.71, 90.00 |
Refinement procedure
| Resolution | 31.290 - 2.000 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k5u |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.290 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | 0.115 |
| Number of reflections | 14476 | |
| <I/σ(I)> | 49.23 | 15.59 |
| Completeness [%] | 92.9 | 69 |
| Redundancy | 5.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298.15 | 25% PEG4000, 0.1M Bicine, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
