3BA3

Crystal structure of Pyridoxamine 5'-phosphate oxidase-like protein (NP_783940.1) from Lactobacillus plantarum at 1.55 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000, 0.9797
Spacegroup name	P 43 3 2
Unit cell lengths	137.379, 137.379, 137.379
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.975 - 1.550
R-factor	0.171
Rwork	0.170
R-free	0.19600
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.684
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.3.0040)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.975	29.975	1.590
High resolution limit [Å]	1.550	6.930	1.550
Rmerge	0.083	0.039	0.913
Total number of observations		7962	43050
Number of reflections	64534
<I/σ(I)>	5.4	12.9	0.9
Completeness [%]	100.0	98.8	100
Redundancy	10.1	9.3	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	NANODROP, 2.0M (NH4)2SO4, 0.2M Li2SO4, 0.1M Tris-HCl pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K